Detail
RTA HSV—1&2 Multiplex Real—Time PCR Kit is a multiplex in vitro nucleic acid amplification assay for the detection of Herpes Simplex Type 1 and Type 2 Virus DNA in human serum samples. The kit is designed to be used as an aid in the management of cure for transplantation patients with potential Herpes Simplex 1 and 2 infection in conjunction with all relevant clinical and laboratory findings.
RTA HSV-182 Multiplex and/or Real-Time PCR Kit provides you everything needed for a reliable and efficient detection:
- Very high sensitivity of detection
- (as few as 63 copies/mL for HSV—l and 58 copies/m1 for HSV—Z)
- Complete specificity
- Whole process check by adding the internal control during extraction
- 2 positive controls
- Compatibility with widely used thermal cyclers
- 98/79/EC EU IVD Directive compliance
RTA HSV-182 Multiplex Real—Time PCR Kit is perfectly designed to amplify and detect highly conserved regions within the HSV-l and HSV-2 genomes by Real Time PCR. It is possible to monitor the nucleic acid presence on—line, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the DNA amount.
Sample Preparation: Nucleic acid extraction is a decisive step prior to qPCR since;
providing high yields of nucleic acid and removal of PCR inhibitors in turn improve the amplification efficiency and critical for avoiding false negative PCR results.
RTA RTA HSV-182 Multiplex Real-Time PCR Kit has been validated for use with RTA Viral DNA Isolation Kit for its best performance.